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Universität Zürich Campus Irchel

190 Winterthurerstrasse

Room Y03-G-85

8057 Zürich

Switzerland

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CRISPR Lunch-Seminar Uni Zürich Irchel

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Optimizing CRISPR ribonucleoproteins to achieve potent, precise genome editing

Speaker: Lai Chan, Field Application Manager - Functional Genomics Integrated DNA Technologies (IDT)

Host: Professor Michael O. Hottiger, Prof. PhD, DVM,

Department of Molecular Mechanisms of Disease

University of Zurich

Abstract

We will review how IDT developed the most potent set of genome editing tools on the market by combining the precision of chemically synthesized guide RNAs with high performance Cas9 and Cas12a (Cpf1) proteins. The Alt-R CRISPR system offers powerful on-target editing and reduced off-target effects compared to traditional approaches. This system is non-toxic and easy to deliver into a wide array of cells by lipofection or electroporation. We will present a novel high-fidelity Cas9 mutant that has been optimized to further reduce off-target gene editing while maintaining on-target potency. In addition, we will discuss a novel Cas12a(Cpf1) mutant with dramatically improved cleavage activity that rivals Cas9 and has improved low temperature cleavage activity making it a universal Cas12a (Cpf1) solution for genome editing in both animal and plant species.

This presentation will review applications, including gene disruption and homology-directed repair (HDR) using both Cas9 and Cas12a systems.

Following the seminar, a free lunch will be served outside the seminar room. We therefore kindly ask you to register for this event.

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Universität Zürich Campus Irchel

190 Winterthurerstrasse

Room Y03-G-85

8057 Zürich

Switzerland

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