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Automated patch clamp: From single channels to 384 giga seals in parallel

Nanion Technologies

Monday, February 17, 2014 at 11:00 PM - Tuesday, February 18, 2014 at 12:30 PM (PST)

San Francisco, CA

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Workshop: Automated patch clamp Ended Free  

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Event Details

 

Workshop on automated patch clamp:

 

from single channels, primary cells, action potentials to 384 giga-seal recordings in a parallel HTS format.

 

 

 

 

 

Biophysics 2014,MosconeCenter,San Francisco,Room:123

 

 

 

Monday,Feb 17, 2014,11:00 AM-12:30 PM


Dr. Niels Fertig, CEO Nanion Technologies

 

Dr. Andrea Brüggemann, CSO Nanion Technologies

 

Dr. Gerhard Baaken, Ionera


 

 

 

 

 

The Port-a-Patch recently turned 10 years old, and is going stronger than ever. It’s still the smallest patch clamp rig in the world, and makes patch clamp recordings accessible to anyone spending a couple of hours with it. Giga-seal recordings and the excellent voltage-clamp of the cellular membrane ensure high quality data, and the Port-a-Patch add-ons allow unprecedented experimental freedom, including temperature control, internal perfusion, automated action potential recordings, and recordings from primary and stem cell-derived cells. Recently, the Port-a-Patch technology was scaled up to 8 simultaneous recordings (Patchliner), maintaining the same data quality and experimental possibilities, and now we did it again: 384 Port-

 

a-Patches have been shrunk to fit inside a shoebox – called the Patch Engine (PE). Two Patch Engines can be integrated per SyncroPatch 384PE platform, allowing for patch clamp-based ion channel HTS from up to 768 cells in parallel, and we’ll tell you more about it during this workshop.

 

 

 

Another topic for the workshop is the bilayer-reconstitution of ion channels and nanopores, efficiently investigated using the Orbit 16, a parallel device for formation of and recordings from up to 16 artificial bilayers at once. Using Micro Electrode Cavity Array (MECA, Ionera), a 4 x 4 array of circular micro-cavities in a highly inert polymer, the bilayer is automatically formed by remotely actuated painting (Ionera- SPREAD).

 

 

 

Welcome to our workshop and learn from live, hands-on experiments on the Port-a- Patch and Orbit 16, and let us show you how to scale up your ion channel screening project to HTS-standards!

 

 

 

Spaces are limited so reserve yours by sending an email to info@nanion.de.


 

Have questions about Automated patch clamp: From single channels to 384 giga seals in parallel? Contact Nanion Technologies

When & Where


Moscone Center
747 Howard Street
Room 123
San Francisco, CA 94103

Monday, February 17, 2014 at 11:00 PM - Tuesday, February 18, 2014 at 12:30 PM (PST)


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Organizer

Nanion Technologies

Nanion Technologies GmbH is a German Private Limited Company and was founded in 2002 as a spin-off from the Center for Nanoscience (CeNS) of the University of Munich. Nanion’s team has developed and globally established two highly successful automated patch clamp instruments as enabling tools for sophisticated and high throughput applications for ion channel research and drug discovery.

Nanion's instruments use planar patch clamp chips which replace the traditional glass pipette used in the technique of patch clamping. Nanion was nominated in 2007 for Germany's most prestigious innovation award the Deutscher Zukunftspreis (German Future Prize, Federal President's Award for Technology and Innovation).

With the Surfer Nanion Technologies progessed also to areas beyond automated patch clamp. for more new technologies check also the Port-a-Patch and Orbit workshop during the Biophysical Society Meeting (previous day same time and place.) or visit us at booth 437.


More Events during the meeting here.

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